Small vessel wire myograph systems

System

Scheme of the system

The system consists of five main units:

1. Organ bath (flow-through/stationary) with buffer in-and outflow stubs (gravitational or pumped flow) gas controller with freely positionable diffuser tip, and field-stimulating electrodes (as per request). Vessel holders: tungsten wires or steel prods (depending on vessel lumen); vessel holder locking for positioning of the organ,
2. In- and outlet stub of the thermostated circulating water bath (20 l, 15 l/min flow rate),
3. Precision manipulator (0.25 mm/turn),
4. Isometric sensor,
5. Base plate with lock/unlock lever for the measuring unit.

 

Organ bath, measuring unit

Organ bath

One-module system with microscope

The organ bath is manufactured in four different volumes (2, 5, 10, 20 ml). The standard volume is 5 ml unless requested otherwise. Each volume is available for later order, and can be replaced in the measuring unit.

The tubes carrying gas and solution for maintaining optimal physiological environment are connected detachably to the measuring unit through connectors fixed in the base plate.
Removable design of the measuring unit greatly facilitates placement of the preparation in the vessel holder because the unit can be put on the stage of a microscope.

 

Removing the measuring unit

 

The organ bath, the tension manipulator and the sensor are built into one unit which can be removed, for the sake of easy handling, in the following way:

Unit locking	1. Turn the lever locking the unit to the base plate and tube connector in 90O angle	Measuring unit and baseplate
Unit sliding	2. Slide out the unit along the guide rail of the base plate

 

Vessel holders, precision setting of gas supply

The tungsten wire vessel holder type ISO-12-21 is recommended for vessel preparations with an inner diameter between 0.05 mm and 0.5 mm, and length up to 3.5 mm.The choice of vessel holders is determined by the inner diameter and length of the preparation. The decision finally depends on the professional competence of the person performing the experiment. The vessel holder can be interchanged in the sleeves, within and between types.

Wire-type vessel holder

The steel prod vessel holder type ISO-12-22 can be used also for preparations with an inner diameter between 0.8 mm and 3 mm, and length over 3.5 mm. This technical solution enables the continuous examination of larger vessels in a small volume of buffer.

Steel spike-type vessel holders

 

Gas controller and diffuser

Gas vaporizer

Gas controller

For proper working with the preparation, it is vital to have the necessary amount and adequate quality of carbogen gas evenly distributed in the solution.
To ensure the sufficient dispersion, a bubbler and fine-tuner device has been developed. The gas coming through the pressure reducer on the gas cylinder is kept in a gas bag. From there, the gas flows in a sufficiently thick-walled tube through a cylindrical clamp and is applied into the solution via a tube-tip gas diffuser.

 

Calibration

Parts of the calibration unit

Calibration is done by means of an independent unit which can be connected to the system.

Parts of the unit:

• Holder with the pulley with bearing,
• Thread with hook,
• 1g certified weight.

 

Calibration

Steps of the calibration:

• Remove the precision manipulator and replace it with the pulley holder,
• Lock the isometric sensor with the lever. Hitch the thread to the stub on the vessel holder sleeve of the sensor, then lead it over the pulley,
• Attach the 1 g calibration weight to the hook of the thread,
• Unlock the sensor by turning the lever back in 90^O.

 

Gravitational flow

Pumped flow

Accessories required for operation

ISO-12W Workstation The analog amplifiers, the computer and the software constitute an autonomous, integrated unit in the workstation. This solution allows fast configuration, training and promotes concentration on the experimental work. The following hardware-software systems are recommended to graphically display, store and analyze the measured physiological parameters: SPEL ISOSYS (MDE GmbH), SPEL KYMOGRAPH (MDE GmbH), LabChart (AD Instruments), AcqKnowledge (BIOPAC), LabScribe (iWorx). ISO-12W	EXP-ST stimulators Square wave stimulator Field stimulation electrode
CWB-02 Circulating water bath Circulating water bath	EXP-SG amplifiers  EXP-SG amplifier family
Ministar peristaltic pump Ministart peristaltic pump	Z-LITE Fiber Optic Illuminator Fiber Optic Illuminator
Surgical tools (RATKIT or individual items) RATKIT	PZMIV-BS Precision Stereo Zoom Microscope Precision Stereo Zoom Microscope