Isolated Nerve Tissue Perfusion setup

MDE provides a reliable and multifunctional research tool for in-vitro nerve tissue experiments. Our in-vitro superfusion release setup enables the collection of the most sensitive neurotransmitters released from nerve tissues during electrical or chemical stimulation.  

The success of our superfusion experiments is attributed to two key factors:

  1. High-Quality Stimulator : Our stimulator is meticulously designed to deliver precise and controlled electrical stimulation to the nerve tissues, ensuring accurate experimental outcomes.
  2. Well-Tested Bath System : Our bath system undergoes rigorous testing to provide optimal conditions for maintaining the health and functionality of nerve tissues throughout the experiment. Additionally, we offer a special bath specifically designed for chemical stimulations, ensuring versatility in experimental setups.

 

Together, these components form a robust platform for conducting superfusion experiments, enabling researchers to gain valuable insights into neurotransmitter release mechanisms and neural function in vitro.

The complete perfusion system includes vital components like the electric stimulator, carbogen gas line distribution, double-walled incubator chambers for the samples, and the perfusion chambers. Electrical field stimulation of the nerve slice can be facilitated using platinum electrodes integrated into the chamber. Additionally, chemical stimulation can be achieved by introducing substances dissolved in the perfusion fluid or directly delivering them to the perfusion chamber, for which we provide resistant materials.

Moreover, the system offers versatility by enabling radioactive labeling of nerve tissues, followed by the release and collection of neurotransmitters for quantitative measurement.

The in-vitro superfusion release technique offers several advantages in neural research: 

  • Temporal Precision: It allows for precise temporal control over the release of neurotransmitters, enabling researchers to study synaptic events with high accuracy and resolution. 
  • Localized Application: The technique permits the localized application of substances, facilitating the study of specific neural pathways and their responses to various compounds. 
  • Minimized Tissue Disturbance: By superfusing substances directly onto the tissue of interest, the method minimizes disturbance to the surrounding environment, preserving the natural conditions for neural activity.
  • Reproducibility: Researchers can achieve consistent and reproducible experimental conditions, enhancing the reliability of results and enabling comparisons across different studies. 
  • Versatility: The technique is versatile and adaptable, allowing for the investigation of diverse neurotransmitter systems and their interactions, contributing to a comprehensive understanding of neural function. 

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